#Cellprofiler nameandtypes error how to#
See the IdentifySecondaryObjects module for details on how to do this. For these reasons, cell bodies are better suited for secondary object identification, since they are best identified by using a previously-identified primary object (i.e, the nuclei) as a reference. In addition, cells often touch their neighbors making it harder to delineate the cell borders. In contrast, cells often have irregular intensity patterns and are lower-contrast with more diffuse staining, making them more challenging to identify than nuclei.These qualities typically make them appropriate candidates for primary object identification. The nuclei of cells are usually more easily identifiable due to their more uniform morphology, high contrast relative to the background when stained, and good separation between adjacent nuclei.We define an object as primary when it can be found in an image without needing the assistance of another cellular feature as a reference. What is a primary object? In CellProfiler, we use the term object as a generic term to refer to an identifed feature in an image, usually a cellular subcompartment of some kind (for example, nuclei, cells, colonies, worms).
Identify Primary Objects identifies biological components of interest in grayscale images containing bright objects on a dark background.